Viewing
a bundle record |
Note: Some of the data shown on this page has been partially modified for display purposes and should not be considered accurate information. Information about genomic elements is displayed in eGenome either as an individual record or as a summary table, depending upon whether one or multiple elements are found in the search. Bundle records have a slightly different format than records for other element types. Bundles are collections of individual elements that share identical database identifiers and compatible genomic positions, and so presumably represent the same genomic feature. An example of a bundle record is shown and explained line-by-line below. Bundle records can be divided into three sections. The top of the page is a bundle data section displaying general information about the bundle and its position in the genome. Below this, the element data section displays individual genomic elements that comprise the bundle. At the bottom of the page is a constant links footer, which consists of a series of external links specific to the element being viewed. |
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Bundle
name: This displays the bundle name selected for display by eGenome.
This name is selected as the most appropriate from a larger group of names
and aliases collected by eGenome representing elements comprising the
bundle. Click here
for an explanation of how names are selected.
Back to Bundle data / Back to top Bundle title: The bundle title is present usually only if the bundle is known to represent a transcript. The title briefly describes the function of the protein encoded by the transcript, if it is known. Characterized transcripts will list known function; uncharacterized transcripts with strong homology to characterized transcripts will indicate a putative function or gene family; transcripts with no known function will often contain non-functional descriptive text only; putative or predicted transcripts will usually indicate that the transcript is not confirmed in some descriptive manner. These titles are taken from UniGene and often are somewhat subjective. Back to Bundle data / Back to top List of Region button: Clicking on this button generates a summary table of all elements within the genomic region that the bundle has been localized to. Back to Bundle data / Back to top Map of Region button: Clicking on this button generates a graphical view of the genomic region that the bundle has been localized within. The graphical view is displayed in Chromoscape. Back to Bundle data / Back to top
Minimal position: Lists the most likely minimal (min) position to which the bundle
is localized. Note that a min position defines the smallest region of
overlap shared by localizations of all elements comprising the bundle.
Information about how min positions are calculated can be found here. In some instances a bundle min cannot
be defined, in which case the minimal position is listed as "none"
and only the max position is shown with a value. These positions are defined
using the genomic sequence positions determined for each element. In essence,
a min position identifies a region of sequence that all elements representing
the genomic object share in common. The first line refers to the sequence range that defines the bundle min position. In the example, the bundle SDR1 has a minimal position defined as between base pairs 12,208,925 and 12,209,060 from the chromosome 1 p terminus. This indicates that all of the elements in the SDR1 bundle include this stretch of sequence. The second line indicates the corresponding cytogenetic position for this region. Back to Bundle data / Back to top
Maximal position: Lists the maximum (max) possible position to which the
bundle is localized. A max position is defined as the combined genomic
region to which any of the elements comprising the bundle localize. Information
about how max positions are calculated can be found here. These positions
are defined using the genomic sequence positions determined for each element.
In essence, a max position identifies the combined region of sequence
that any of the elements representing the genomic object may have. The first line refers to the sequence range that defines the bundle max position. In the example, the bundle SDR1 has a maximal position defined as between base pairs 9,194,835 and 14,296,882 from the chromosome 1 p terminus. This indicates that for any portion of this region, one or more of the elements in the SDR1 bundle is localized there. The second line indicates the corresponding cytogenetic position for this region. Back to Bundle data / Back to top Help button: This help button links to the bundle record help page that you are currently viewing. |
| Element data |
SNP symbol: This square-shaped symbol will appear directly after any element name in the Name column of the summary table that contains an identified SNP somewhere within the sequence that the element spans. Back to Element Data / Back to top RH marker & polymorphism symbol: This star-shaped symbol will appear directly after any element name in the Name column of the summary table that represents both an RH marker and a polymorphism. These elements have both genetic linkage and RH positional data associated with them and have been independently localized by each technique. Back to Element Data / Back to top Bold text: A statement "Bold text - RH/GL framework element" will appear in the symbols legend whenever one or more elements in the summary table are members of the framework maps used to construct the overall RH and genetic linkage maps (click here for details of this process). Any such elements have their entire entries displayed in bold text. Back to Element Data / Back to top Name column: Lists the names of all elements comprising the bundle. This displays the official element name selected for display by eGenome. The element name is selected to be most appropriate from all of names and aliases that the eGenome database has identified. Click here for an explanation of how names are selected. The element names are clickable and link to their corresponding individual records. For example, clicking on "SHARP" in the first entry shown above would give more complete information about that element. In many cases, including all elements listed in the example above, multiple listed elements share the same name. This is because elements with the same name, but with certain different characteristics (such as different primer sequences), have been independently localized. Back to Element data / Back to top Status column: Indicates whether the genomic element represents a sequence feature that generates an RNA transcript. The status options are transcribed, not transcribed, and unknown. Back to Element data / Back to top Sequence position column: Lists the position of the genomic element in the NCBI DNA sequence assembly. The number indicates the base pair position of the sequence stretch that the element comprises, rounded to the nearest kilobase. If no position was found, the entry reads "Unknown". If there are multiple sequence positions, the entry reads "Multiple", and all of the reported positions can be viewed in the element record by clicking on the element name. More information about why multiple or discrepant sequence positions may occur can be found here.The base pair positions are calculated beginning at the pterminus of the chromosome (bp position 1 is the pterminal-most base that has been determined for that chromosome). These sequence positions have been determined using e-PCR, and a sequence position is reported only if both primer sequences for the genomic element exactly match the genomic sequence with the proper distance and orientation between each primer. See the Methods section for information about how the sequence positions are calculated. Back to Element data / Back to top RH position column: Lists the RH framework position or interval position of each element, usually defined by two flanking RH framework markers. If an element is or localizes near a single RH framework marker, only that single framework marker is shown. See the Methods section for information about how the RH positions are calculated. Back to Element data / Back to top Cytolocation column: Lists the cytogenetic band or band range that has been calculated for the displayed element's cytogenetic position. See the Methods section for information about how the cytolocations are calculated. |
| Links footer |
At the bottom of the page are a series of buttons that allow the user to search for the genomic element being viewed in a number of external databases. In each case, this performs a search in the linked database using the eGenome element name. As each database contains different sets of data and identifiers, these searches often find no records or multiple records in the database being searched. As such, these results may require further investigation of the external site. Search specifier: This phrase indicates the search term that is used to represent the genomic element (usually a gene symbol, SNP name, or marker name) when searching the various databases. Back to Links footer / Back to top UCSC: Searches the UCSC genome browser and displays either a list of matching records or, if a single exact match is present, a graphical representation of the element's genomic region. Back to Links footer / Back to top Ensembl: Searches the Ensembl genomic catalog and displays a list of records matching the element name. Back to Links footer / Back to top NCBI MapViewer: Searches the NCBI MapViewer genome browser and displays a chromosome graphic showing where elements with this name map, along with a list of matches below the graphic. Click on list members to view a graphical representation of the element's genomic region. Back to Links footer / Back to top GenBank: Searches the GenBank DNA sequence repository and displays a list of DNA sequence records containing the element name. For a more specific search of GenBank records, use the direct sequence accession links in the Clones & Sequences tab. Back to Links footer / Back to top GDB: Searches the Genome Database genomic repository and displays either a table of matching records or, if a single exact match is present, the record matching the element name. Back to Links footer / Back to top GeneCards: Searches the GeneCards gene catalog and displays a table of records matching the element name. Elements that do not represent transcripts are not included in GeneCards. Back to Links footer / Back to top Entrez Gene: Searches the NCBI Entrez Gene gene catalog and displays a list of records matching the element name. Elements that do not represent transcripts are not included in Entrez Gene. As Entrez Gene includes several species, non-human elements may also be included. Back to Links footer / Back to top UniSTS: Searches the NCBI UniSTS genomic marker catalog and displays a list of records matching the element name. As UniSTS includes several species, non-human markers may also be included. Back to Links footer / Back to top Swiss-Prot: Searches the ExPASy Swiss-Prot protein database and displays a list of protein records corresponding to transcripts matching the element name. Elements that do not represent transcripts are not included in Swiss-Prot. As Swiss-Prot includes several species, non-human proteins may also be included. Back to Links footer / Back to top HomoloGene: Searches the NCBI HomoloGene nucleotide homology database and displays a list of transcripts with strong sequence homology to the element being viewed. Elements that do not represent transcripts are not included in HomoloGene. As HomoloGene includes numerous species, non-human transcripts may also be included. Back to Links footer / Back to top OMIM: Searches the NCBI OMIM human genetic disease locus database and displays a list of known and putative human disease loci in some way associated with the element being searched with. Note that this search looks for the presence of the search term in all OMIM records. Because of this, matches may mention the search term in the OMIM narrative without actual disease implication. Back to Links footer / Back to top PubMed: Searches the National Library of Medicine MEDLINE biomedical literature database and displays a list of manuscript records matching the element name. Back to Links footer / Back to top BioHunt: Searches the ExPASy BioHunt molecular biology-specific Internet search engine and displays a list of catalogued web pages on the Internet that contain the element name. Back to Links footer / Back to top Internet: Searches the Google Internet search engine, which displays a list of web pages containing the element name. Back to Links footer / Back to top
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| Except as otherwise indicated, Copyright 2005, The Children's Hospital of Philadelphia |